Mutagenic Activities of Aromatic Amines in Salmonella typhimurium and its Effect on Rat Drug Metabolizing Enzymes PDF

Keywords

amines, Salmonella typhimurium, rat drugs, enzymes

Abstract

The aromatic amines including o-toluidine, 4-chloro-o-toulidine, 4-4'-methylene dianiline (MDA), 4, 4'-methylene bis (2- chloroaniline) (MOCA) and its three possible substitutes, ethacure 300, cyanacure, and polacure 740 M, were used to determine the mutagenic activities in Salmonella typhimurium strains TA98 and TA100. The mutagenic activities of these chemicals were compared with their effects on ethoxyresorufin o-deethylase (EROD) activity and aldrin epoxidase (AE) activity in rat liver. The chemicals o-toluidine, 4-chloro-o-toluidine, MDA, MOCA and its substitutes, ethacure 300 and cyanacure, showed mutagenic activities, while polacure 740 M showed no mutagenic activity. All the mutagens caused an increase in EROD activity, while polacure 740 M showed no appreciable increase in EROD activity. Thus, there was excellent correlation between mutagenicity and EROD induction. This supports the conclusion that the ability of a chemical to induce EROD bears some relationship to its carcinogenic potential.