The Effects of Apelin on Myocardial Function after Resuscitation of Hemorrhagic Shock in Rats PDF

Keywords

Apelin, Myocardial Function, Hemorrhagic shock, Sprague Dawley rats, Langendorff.

Abstract

Apelin is a novel peptide that has recently been established as the only known ligand for the APJ receptor. Apelin has an important regulatory role in cardiovascular homeostasis. Despite recent advances in the understanding of the cardiovascular effects of the apelin-APJ system, the myocardial protective effects of treating with apelin before resuscitation following hemorrhagic shock has not been investigated. The present study investigated the myocardial protective effects of apelin on preventing myocardial contractile dysfunction after hemorrhagic shock. Methods: Male Sprague Dawley rats (300-350 gm) were assigned to 3 experimental groups (n= 6 per group): Normotensive rats (N); Hemorrhagic shock rats (HS); Hemorrhagic shock rats treated with apelin-13 (HS-AP). Rats were hemorrhaged over 60 min to reach a mean arterial blood pressure of 40 mmHg. Rats were treated with 1 ml of 10 nm /L apelin-13 intra-arterially after 60 min hemorrhagic shock. Resuscitation was performed in vivo by the reinfusion of the shed blood for 30 min to restore normo-tension. Left ventricular contractile function was measured in the isolated hearts following hemorrhage and in vivo resuscitation using the Langendorff apparatus. Results. Hemorrhagic shock rats treated with AP exhibited a significant increase in left ventricular generated pressure LVGP (111.20 ± 9.19 mmHg) and + dP/dtmax (589.6 ± 110.68 mmHg/sec) compared with the untreated group. Conclusion.Treatment with apelin before resuscitation improved myocardial contractile function in a hemorrhagic shock model in rats.