Transformation of Cortisone-21 Acetate with Streptomyces atro olivaceus PDF

Keywords

acetate, enzymes, transformation process

Abstract

The enzymatic hydrolysis and ∆1- dehydration of cortisone-21-acetate with S. atro olivaceus were evaluated. Highest yields of prednisone were obtained when the transformation process was conducted using cells in their logarithmic growth phase (60 hr old culture). optimal conversion estimates of cortisone-21-acetate were obtained with 5 mg substrate/2 g cell fresh weight/50 ml reaction mixture. Supplementation of the reaction mixture with adenine and adenosine retarded the formation of prednisone while menadione and riboflavin led to the formation of high yields of prednisone. Similarly, Fe++ and Mg++ encouraged the formation of high yields of prednisone while Mn++ gave comparatively lower product yield.