Frequency of Juxtaglomerular Granulated Cells in Diuretic (Frusemide) Treated Mice PDF

Keywords

furosemide, albino mice, water

Abstract

The present study was carried out to elucidate the effects of furosemide on the degree of granularity of the JGCs. Fifteen male young-adult albino mice were given 0.3 mg/ml furosemide in their drinking water. Each experimental mouse was found to have drunk 10-12 ml of water containing furosemide, while control animals drunk 2-3 ml of drinking water. After three weeks, all animals were sacrificed and kidneys were fixed in 10% buffered formalin and processed for light microscopy. Sections were stained for juxtaglomerular cells (JGCs) demonstration by alkaline crystal violet (Harada 1971) and by Bowie's method (Bowie 1936). The juxtaglomerular index (JGI) and the percentage of granulated nephron (%GN) were calculated according to the method of Hartroft and Hartroft (Hartroft & Hartroft 1953). Kidneys from experimental animals showed an increase in both JGI and %GN following treatment with furosemide indicating hypertrophy, hyperactivity of the JGCs and increasing rate of renin release.