Correlation Between Gel Electrophoretic Profile of Bacterial Lipopolysaccharide and its Toxicity PDF

Keywords

toxicity, lipopolysaccharide, gel electrophoretic

Abstract

Gel electrophoretic pattern of lipopolysaccharide (LPS), prepared from Pseudomonas aeruginosa isolates, revealed variable banding, assuming quantitative and qualitative differences leading to three main distinctive profiles. The first is a ladder-like banding arrangement of molecules, with various oligosaccharide repeating units and variable affinity to the silver stain (lightly and deeply stained bands). The second banding pattern consists of profiles which are characterized by aggregates of molecules with homogenous oligosaccharide repeating units and forming largely deeply stained bands. The third banding patterns are those having limited number of bands running to the far end of the lanes and staining differently. The toxicity of the studied LPS is closely related to the number of deeply stained bands.