Analysis of Onion Seeds by Two Dimensional Electrophoresis PDF

Keywords

Egypt, onion seeds, protein buffer extract

Abstract

Egyptian onion seeds (Giza 1 and Shandawel) were extracted with tris-borate buffer solution of pH 8.9. The protein buffer extract was examined by two dimensional electrophoresis (Mapping technique); with PAGIF in the first dimension, and Poro PAGE and SDS-PAGE in the second dimension. Differences between the cultivars, stored and fresh onion seeds could be detected.