Studies on Camel Casein Micelles Treatment with Soluble and Immobilized Pepsin PDF

Keywords

camel casein micelles, pepsin, nitrogen

Abstract

Isolated camel casein micelles were treated with pepsin, in both soluble and immobilized forms, to study the location and distribution of glycosylated portion (glyco-к-casein-like component) of camel casein micelle. The release of nonprotein nitrogen and sialic acid soluble in 2% and 12% trichloroacetic acid (TCA) was studied. Soluble pepsin released a maximum amount of 5.62 and 4.97 mg NPN/g casein soluble in 2% and in 12% TCA, and 7.1 mg sialic acid/g casein soluble in 2% or 12% TCA, respectively. The corresponding figures for immobilized pepsin were 4.97, 4.30 and 6.7. It was concluded that almost (90%) of glycosylated portion of camel casein micelle is on the micelle surface.