Farah Shireen, Bashir Ahmad, Saad Ahmad Khan, Abdur Rauf, Anees Ahmed Khalil, Fawad Aziz, Abdel Rahman Al-Tawaha, Mohammed A. Al-Duais, Yahya S. Al-Awthan, Gokhan Zengin
Article publication date: 2022-06-29
Vol. 39 No. 4 (yearly), pp. 283-302.


Agave americana, Mentha spicata, Mangifera indica, biological screening.


Purpose: The research study was prototyped to assess pharmacological activities (antimicrobial, anti-oxidant, phytotoxic and haemagglutination) of Agave americana, Mentha spicata and Mangifera indica leaves extracts. The positive outcomes of the study can aid in formulations of novel therapeutic opportunities by using natural, eco-friendly, and economic raw materials having least side effects and aced potency. Method: Leaves extracts of Agave americana, Mentha spicata and Mangifera indica were prepared using analytical grade ethanol, methanol, acetone and n-hexane. Then the extracts were subjected to antibacterial, antifungal, antioxidant, phytotoxic and haemagglutination assay. Well-diffusion antibacterial protocol was followed against pathogenic bacteria. Tube dilution antifungal protocol was followed against pathogenic fungi. DPPH radical scavenging assay at variable sample dilutions was followed for antioxidant assay. Lemna minor lethality assay was followed for phytotoxic assay. Haemagglutination assay were assessed against ABO blood groups. Results: Crude leaves extracts (aqueous, acetone, methanolic and ethanolic) of preferred plants i.e., Agave americana, Mentha spicata and Mangifera indica owned superlative antimicrobial efficacy against all test micro-organisms specifically Escherichia coli ((91%), Pseudomonas aeroginosa (85%), Methicillin resistant Staphylococcus aureus (81%) and mycelium of Fusarium oxysporum (85%) was halted by Agave americana leaves extracts. In comparison to these crude extracts of Mentha spicata terrifically halted growth of Proteus mirabilis (80%) while leaves extracts Mangifera indica halted growth of Streptococcus griseus (90%) respectively. Excellent antioxidant and phytotoxic efficacy were recorded at higher sample concentrations. No haemagglutination efficacy recorded. Conclusion: From the study it was concluded that leaves of Agave americana, Mentha spicata and Mangifera indica possess excellent antimicrobial potentials which can aid to formulation new antibiotics having excellent effects in least doses. These extracts can also be used to treat many maladies by eliminating reactive oxidants and promoting healthy metabolism. These extracts can also be utilized as ecofriendly and user-friendly herbicide.