Analyses of the Carbohydrates Leached During Drying and Wetting of Sclerotia of Sclerotinia sclerotiorum (Lib) De Bary PDF

Keywords

sclerotia, carbohydrates, leaching

Abstract

Sclerotia of Sclerotinia sclerotiorum (Lib) De Bary were exposed to different humidities ranging from zero to 100% for different periods of time up to 168 hr. Leaching of carbohydrates occured after sclerotia were wetted. At zero and 33% relative humidities leaching of carbohydrates was highest and increased with period of exposure. Quantitative estimation of carbohydrates released showed that leaching was in the order trehalose, mannitol, glucose with fructose in trace amounts. On drying and rehydration of sclerotia at two relative humidities (zero and 100%) severe carbohydrate leaching occurred. Quantities leached were found to increase with the period of incubation at zero relative humidity but decreased with the period of incubation at 100% relative humidity.