Evaluation of Two Insect Chemosterilants (Hampa and Thiotepa) for their Effect on Eimeria vermiformis Infection in Mice PDF

Keywords

mice, infection, Utah

Abstract

The mice used in this study were a total of 42; they were divided into seven experimental groups. Each mouse was inoculated with 20,000 sporulated oocysts of Eimeria vermiformis. The results showed that hempa and thiotepa exert an effect on the mice bearing E. vermiformis when administered orally at the rate of 0.33 mg per mouse. Doses were calculated from the LD50 data for each of the drugs in mice. The doses of hempa apparently caused increased oocyst production. Peak oocyst discharge and patent period were found to be delayed in the treated groups. The doses of thiotepa was kept the same in all animals, but the time of administration was synchronized with the developmental stages of the parasite. It was found that by gradually increasing the duration of the drug administration, oocyst discharge was increased. Both drugs may affect the immune system of the mice and the recognition of antigen of the parasite. It could be useful to use these immunosuppressive agents to prolong the retention of transplant tissues in the body.