Author(s): Majed Hussain Al-Gelawi and Oday Adnan Al-Makadci
Article publication date: 2007-06-01
Vol. 25 No. 1/2 (yearly), pp. 16-22.
190
Keywords
Pseudomonas aeruginosa, Mutagenesis, Rhamnolipid
Abstract
Six bacterial isolates (isolated previously) were identified and/or ensured their identification Results showed that these isolates belong to P aeruginosa, and all isolates were capable of producing rhamnolipid, and the best one was P Aeruginosa RB67. In order to get rhamnolipid hyper producer mutants, mutagenesis of P aeruginosa RB67 using UV light and MNNG were performed. Fifty colonies from each treatment (UV and MNNG) were selected and screened for their ability to produce rhamnolipid semi-quantitatively by replica plated on blood agar and CTAB-methylene blue agar Based on the last method, twelve colonies from each treatment (UV and MNNG) were selected and used for measuring rhamnose concentration The results showed that these mutants varied in their ability to produce rhamnolipid, and some of them showed an increase in rhamnolipid production. The highest rhamnose concentration (94 ug/mL) was achieved by the mutant (MOM12). Furthermore, FTIR spectroscopy results indicated that there were no apparent qualitative differences in rhamnolipid produced from mutants.