Semen Cryopreservation using Triladyl and Tris Diluents of the Arabian and Gazelle Males (Gazella subgutturosa marica) PDF

Keywords

Semen, cryopreservation, Arabian Sand Gazelle, Triladyl, Tris

Abstract

The Arabian Sand Gazelle Males (Gazella subgutturosa marica) is one of the endangered species in Saudi Arabia. The assessment of its reproductive efficiency and semen characters have not been deeply investigated. The present study focuses on this issue, and investigates proper methods of semen dilution and freezing. The study indicated the suitability of the electro-ejaculation as a method of semen collection. Freezing of the semen at - 196°C in plastic straws (0.25ml) is the most common method for semen preservation. Two semen diluents were used; the first was a commercial diluents (Triladyl) and the other was prepared in the laboratory (Tris) diluents. When the average of individual motility before freezing was compared with that after thawing, it was found that a large number of spermatozoa was significantly (P<0.01) adversely affected by freezing. There was also significant difference (P<0.01) between individual post thawing motility of the Triladyl-diluted frozen semen and that of the Tris-diluted one during October and November. Therefore, the best results were obtained when Triladyl was used for extending the Arabian Sand Gazelle semen using freezing by liquid nitrogen rather than Tris diluents. It is recommended that the use of captive population program with cryopreservation of genetic resources bank would be suitable for the preservation and protection of the endangered species, such as the Arabian Sand Gazelle (Arabian Oryx)