PCR Cloning of Polyhydroxybutyrate Synthase Gene (phbC) From Aeromonas hydrophila PDF

Keywords

Cloning Polymerase Chain Reaction (PCR), Poly-hydroxybutyrate, Aeromonas hydrophila, Bacteria, Biodegradation, Plastics.

Abstract

Plastic wastes are considered to be severe environmental contaminants causing waste disposal problems. Widespread use of biodegradable plastics is one of the solutions, but it is limited by high production cost. A polymerase Chain Reaction (PCR) protocol was developed for the specific detection and isolation of full-length gene coding for polyhydroxybutyrate (PHB). (PCR) strategy using (PHB) primers resulted in the amplification of (DNA) fragments with the expected size from all isolated bacteria. (PHB) synthase gene was cloned directly from Aeromonas hydrophila genome for the first time. The cloned fragment was named (phbCah). The nucleotide sequence of (phbCAh) gene exhibits similarity to (PHB) synthase genes of Alcaligenes latus and Pseudomonas oleovorans (97%). Alcaligenes sp. (81%), and Comamonas acidovorans (84%).