Cytokine Gene Polymorphism in Bahraini Patients with Coronary Heart Disease PDF

Keywords

Atherosclerosis, TNF-alpha, IL-6, IFN-gamma, IL-10, TGF-beta1, SNPs

Abstract

There is increasing evidence that inflammatory processes play a central role in the pathogenesis of atherosclerosis. studying the genetic variants of the inflammatory system components in atherosclerosis is very important because it might explain the diverse and variable inflammatory responses among individuals. We herein investigated the possible association between polymorphisms in the pro-inflammatory cytokines tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-6 and interferon-gamma (IFN-gamma), and the anti-inflammatory cytokines (IL-10 and transforming growth factor-beta1 (TGF-beta1), and correlated them with the severity of cardiovascular complications involving arterial coronary heart disease in 28 Bahraini patients, recruited from Bahrain Defense Forces Hospital (BDFH). Twenty nine asymptomatic subjects were randomly selected from blood donors, and were used as negative controls. For each study object (patients and controls), blood specimen collected and genomic DNA was extracted. Genotyping was performed by SSP method using Cytokine Genotyping Tray kit, followed by electrophoresis. The prevalence of the polymorphisms of all studied cytokines in these patients revealed no significant difference in comparison with the apparently healthy normal control subjects (TNF-alpha p< 0.3735, IL-6 p< 0.2863, IFN-gamma p<0.4222, IL-10 p<0.462, TGF-beta1 p<0.0741). Thus, cytokine genes may not play significant role in coronary heart disease process, as their actual effective protein.