Genomic DNA Extraction from Various Tissues of Prawn Penaeus semisulcatus: A Modified Procedure PDF

Keywords

Penaeus semisulcatus, Tissues and organs, molecular, genomic, DNA, Arabian Gulf, Kuwait

Abstract

The objective of this study was to adapt a procedure isolate genomic DNA from the prawn Penaeus semisulcatus collected from the Arabian Gulf region. Different tissues and organs of the prawn were used for the isolation of biologically active, intact gDNA. Several attempts to isolate DNA from the prawn Penaeus semisulcatus using previously reported procedures proved of limited success. Subsequently, a modified protocol for the extraction of total cellular DNA from various tissues of the prawn was developed and optimized. Here, it is demonstrated that the modified DNA isolation procedure is suitable for Penaeus semisulcatus species, and yields intact, pure gDNA that is suitable for molecular studies.