Fungal Species, Go-T Enzyme and Mycotoxins Isolated from Decayed Sugarcane (Saccharum Officinarum) from Qena Governorate, Egypt PDF

Keywords

Egypt, Qena, Sugarcane, Fungi, aquatic and terrestrial fungi, Saccharum officinarum, mycotoxins, enzymes.

Abstract

Seventy-three fungal species belonging to 43 genera were isolated from 40 samples of Saccharum officinarum collected from Naage-Hamadi canal in Qena Governorate) on 10g/l glucose (20 species, 15 genera) in Winter and (23/15) in Summer and on 40 g/l sucrose (42/28) in Winter and (27/18) in Summer on Czapek's agar media at 25°C. Aspergillus, Trichoderma, Mucor and Pythium were the most common genera on the two isolation media. The dominant of Aspergillus were A niger, A. flavus, Aulus, A. Terrens and A. wentii. Some species were dominant on 40 g/l sucrose such as Aspergillus niger, A. flavus, Emericella nidulans, Trichoderma viride, Torula herbarum and Mamana echinoeotryoides, while the dominant species on 10 g/l glucose were Mucor circinelloides, Aspergillus niger Torula herbarum and Trichoderma viride. Mycotoxins including aflatoxins B1, B2, G1 and G2, zearalenone and diacetoxyscirpenol were detected in the examined samples of Saccharum officinarum. The mycelial growth of Aspergillus flavus, A. niger, Fusarium moniliforme and Torula harbarum decreased with the increase in Dimethoate concentrations, although 25 ppm was less effective than the higher levels of the insecticide (75 - 200 ppm). Dimethoate stimulated the activity of Go-T in Aspergillus niger, Fusarium moniliforme and Torula harbarum, while the Go-T activity inhibited in A. flavus with the Dimethoate treatments.