A Comparative Study on Isolation, Culture and Regeneration of Cell Suspension Protoplasts of Solanum Tuberosum L. PDF

Keywords

Plant regeneration, potato, protoplasts, Solanum tuberosum, suspension culture

Abstract

Protoplasts were isolated from stem-derived cell suspensions of S. tuberosum cvs. Desiree and Maris Piper using three enzyme mixtures. The enzyme mixture containing 1% cellulase ("Onozuka" R-10) and 0.1% Pectolyase Y-23 in 0.5 M Mannitol with an incubation period of 3h resulted in high protoplast yield. Isolated protoplasts were cultured in different media. In MS-KM liquid medium, they divided and formed small cell colonies. Resulting calluses were transferred either to MS medium containing 0.05 µM NAA, 2.85 µM zeatin riboside and 0.03 µM GA8 or to modified MS medium containing 0.54 µM NAA, 0.29 µM IAA, 2.22 µM BAP, 2.28 µM zeatin, 0.93 µM kinetin and 0.58 µM GA3 for shoot regeneration. Shoot regeneration was 2 weeks earlier on the former medium.