Existence of Soil Microflora Producing Amylases and Proteases in Eastern Region of Saudi Arabia PDF

Keywords

soil samples, Saudi Arabia, microorganisms, protease, amylase

Abstract

Soil samples were collected from different location in Eastern region of Saudi Arabia in an attempt to isolate protease and amylase producing microorganisms. The soil dilution plate method was used for the isolation of microorganisms. Modified glucose yeast extract agar medium supplemented with gelatine (GYACc) and starch (GYAS) was used. Thermophilic and mesophilic bacteria and mesophilic fungi producing protease enzymes were isolated in all location. No thermophilic actinomycetes and fungi producing the enzyme were recovered in any of the localities. The same pattern was observed in case of recoveries of amylase producing microorganisms. Species of thermophilic actinomycetes were recovered from soil of Dammam, Qatif and Safwa. Thermophilic fungi were also recovered from three localities (Dammam, Sayhat and Thoqbah). No thermophilic microflora producing protease and amylase enzymes were obtained from Abqaiq and Jubail soils. Among the counts of protease producing organisms, Bacillus sp 2 was dominant in 9 localities. It was absent from Abquiq soil. Streptomyces sp 2 was found in Dhahran, Dammam, Naieria localities. Penicillium sp 1 was commonly found in Abquiq, Jubail, Hassa, Naieria, Qatif, Sayhat and Thoqbah localities. Bacillus sp 2 as amylase producing was found in 8 localities. Streptomyces sp 1 and sp 2 were found from the same five localities (Abquiq, Dammam, Dhahran, Naieria, and Qatif). Penicillium sp 1, Aspergillus ap 2 and Mycelia Sterilia were recorded in four different localities for each.