Author(s): A A Hafez and A H Bello
Article publication date: 2002-06-01
Vol. 20 No. 2 (yearly), pp. 101-105.
140
Keywords
Bud formation, Leaf segments, Bellis Perennis, Borago officinalis, Auxins, Cytokinins, concentration
Abstract
Adventitious bud formation was achieved from leaf segments of two important medicinal plants: Bellis perennis and Borago officinalis on Murashige and Skoog's medium supplemented with a various concentrations of Auxins and Cytokinins. In the case of Bellis perennis: high regeneration rate of 7.5 bud/ segment was obtained from each leaf segment on medium containing 2 mg^-1 of BAP and 1 mg^-1 of IBA. Regenerated buds from leaf segments were multiplicated on new media. Proliferation rate of buds was 13.2 bud/sample in presence of 1 mg^-1 BAP and 0.1 mg^-1 IAA. Rooting of some regenerated buds were established on medium containing 0.5 mg^-1 of IAA. The rooted plantlets were successfully transferred to the soil. In the case of Borago officinalis: the regeneration rate of bud/segment was obtained on medium containing 2 mg^-1 of BAP and 0.5 mg^-1 of IAA. Low proliferation rate of buds was obtained (3 bud/sample).