Comparison of the in vitro Fertilization of Ova Obtained from Two Mouse Strains (Balb/c and C57) by Three Different Methods and Cultured in Four Different Culture Media PDF

Keywords

Ova, mouse strains, in vitro fertilization

Abstract

In this study a comparison was carried out between two mouse strains (Balb/c and CS7) on the in vitro fertilization of ova obtained from females by three different methods: regular mating, pseudopregnancy and superovulation. The ova were cultured in four types of media: two commercially obtained media (M2 and M16 from Sigma Company, U.S.A.) and two laboratory prepared modified culture media, (MCM) and KSOM. Balb/c females gave more ova than CS7 ones. Superovulation resulted in significantly (P < 0.05) more ova than any of the other two methods. CS7 females gave more abnormal ova than did Balb/c ones, but their ova were more resistant to unfavourable conditions when cultured in vitro than those of Balb/c females. Balb/c ova obtained by regular mating developed better in MCM (73%) and in M2 media (55%) than in MI6 (6%) and KSOM (5%) media. Similarly, (CS7) ova obtained by the same method have developed better in MCM (55%) and M2 (50%) than in MJ6 (11%) and KSOM (27%) media